European Psychiatry - Volume 33 - March 2016

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S344 24th European Congress of Psychiatry / European Psychiatry 33S (2016) S116–S348
(immunohistochemistry) with their corresponding murine anti-
body and viewing through optical microscope.
Results Lower immunoreactivitywith the antibody anti-TH in the
animals treated with paroxetine, at all levels of the dopaminergic
activity (tracks mesolimbica, cortical circuit, nigrostriatal pathway
and tubero-infundibular). This decrease is reaffirmed after the sta-
tistical treatment of data.
Conclusions Treatment with paroxetine in animal models causes
a depletion of the dopaminergic system that can be one of the
biological bases of sexual dysfunction, altering the reward mecha-
nisms as well as producing hyperprolactinemia.
Disclosure of interest The authors have not supplied their decla-
ration of competing interest.
EW650
Protective effect of curcumin on
diazepam-induced behavioral
changes and oxidative stress in rats
A. Sevastre-Berghian ∗, V. F˘ag˘ar˘as¸ ˘an , N. Decea , R. Moldovan ,
B. Sevastre , M. T˘aulescu , A.G. Filip
1 University of Medicine and Pharmacy “Iuliu Hatieganu”, Physiology,
Cluj-Napoca, Romania
2 University of Agricultural Science and Veterinary Medicine, Faculty
of Veterinary Medicine, Pathophysiology, Cluj-Napoca, Romania
3 University of Agricultural Science and Veterinary Medicine, Faculty
of Veterinary Medicine, Morphopathology, Cluj-Napoca, Romania
∗ Corresponding author.
Introduction Curcumin (CUR), a polyphenolic compound,
extracted from Curcuma longa, is known for its neuroprotective,
antioxidant and anti-inflammatory effects.
Objectives To evaluate the effect of CUR on ambulatory activity,
spatial working memory and on oxidative stress in rats induced by
Diazepam (DZP) administration.
Aims To analyze whether CUR may improve the cognitive per-
formance and offer systemic protection from oxidative stress.
Methods The effect of CUR on DZP-induced memory impairment
and oxidative stress was studied on Wistar rats. Group I received
a vehicle, group II – vehicle and CUR, group III – vehicle and DZP,
group IV – vehicle, CUR and DZP. CUR (150mg/kg bw) and vehicle
were orally administered for fiveweeks long. DZP (2mg/kg bw) was
administered i.p. 20minutes before the behavioral tests. Behavioral
tests, i.e. Open Field and Y Maze Test, were performed. Malondi-
aldehyde and reduced glutathione/oxidized glutathione ratio were
determined in the serum and brain tissue homogenate. Hippocam-
pal sectionswere histologically assessed. The datawere statistically
analyzed by one-way ANOVA, followed by Dunns post-test.
Results DZP decreased (P < 0.01) the number of spontaneous
alternations, as compared to control group, thus suggesting an
impairment of spatial working memory. Behavioral tests revealed
no enhancing effect of CUR on spontaneous alternation behaviors
in Y Maze. CUR reversed (P < 0.01) the inhibitory effect of diazepam
(P < 0.05) on the ambulatory activity in OFT and decreased the lipid
peroxidation in the serum (P < 0.05).
Conclusions The results show that CUR may offer systemic
protection from oxidative stress, thus improving the cognitive per-
formance.
Disclosure of interest The authors have not supplied their decla-
ration of competing interest.
EW651
Right prefrontal deep TMS effects on
attention symptoms: Behavioral
outcomes and electrophysiological
correlates
H. Shahar ∗, U. Alyagon , A. Lazarovits , A. Hadar , D. Cohen ,
H. Shalev , A. Zangen
1 Ben-Gurion University of the Negev, Sede Boqer, Israel
2 Ben-Gurion University of the Negev, Life sciences, Sede Boqer, Israel
∗ Corresponding author.
Introduction The validated treatment for ADHD is chronic admin-
istration of psychostimulants, which is associated with side effects
and occasionally not tolerated. Deep TMS using special coil designs
for targeting neural networks linked with ADHD, may become a
potential alternative.
Objectives Comparison of rTMS treatment using deep, figure-8
and sham coils on ADHD symptoms.
Methods In the current randomized, sham-controlled study,
adult ADHD patients (n = 30) received 15 daily sessions of high-
frequency rTMS directed to the right PFC, using either deep,
figure-8, or a sham coil. ADHD symptoms and cognitive alterations
were assessed using the CAARS-INV, self–report questionnaires and
performance tests. Additionally, the stop signal task (SST) com-
binedwith EEGmeasures was used to assess behavioural inhibition
and ERPs. EEG responses to an inhibitory protocol of paired TMS
pulses over the rPFC were measured before and after treatments.
A healthy control group was evaluated at baseline for comparison
(n = 39).
Results dTMS treated patients (but not standard figure-8 or
sham) showed improvement of ADHD symptoms. Differences
between ADHD patients and healthy controls were demonstrated
in N200 and P300 components of ERP during the SST, and in
response to single and paired TMS pulses. The amplitudes of ERPs
in patients correlated with ADHD symptoms and behavioural inhi-
bitionmeasures. Neurophysiological measures were found to serve
as predictors for effectiveness of dTMS treatment.
Conclusions Repeated stimulation of deep areas in the rPFC has
therapeutic potential in ADHD patients. Ongoing analysis attempts
to evaluate the extent of these findings and establish biomarkers
for treatment efficacy.
Disclosure of interest The authors have not supplied their decla-
ration of competing interest.
EW652
The F-Multidimensional
Perfectionism Scale-18 (FMSP-18):
Internal consistency, construct,
concurrent and divergent validity
M.J. Soares ∗, A.T. Pereira , A. Araújo , D. Silva , A.P. Amaral ,
J. Valente , N. Madeira , M. Bajouco , A. Macedo
1 Faculty of Medicine, University of Coimbra, Department of
Psychological Medicine, Coimbra, Portugal
2 Institute Polytechnic of Coimbra, College of Health Technologies,
Coimbra, Portugal
∗ Corresponding author.
Introduction The FMPS is a 35-item self-report questionnaire to
measure perfectionism. It evaluates: concern over mistakes/CM,
doubts about actions/DA, parental criticism/PC and expecta-
tions/PE, personal standards/PS and organization/O.
Objectives To develop a shortened version of FMPS and study
its internal consistency, the construct, concurrent and divergent
validity.
Methods One hundred and ninety-two university students
(78.1% females), aged 19.74 years (sd = 2.10) completed the