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24th European Congress of Psychiatry / European Psychiatry 33S (2016) S18–S55

S31

2

Hôpital R.-Debré, Psychiatrie de l’enfant et de l’adolescent, Paris,

France

3

Hôpital Sainte-Anne, CMME, Paris, France

Corresponding author.

Introduction

Anorexia nervosa (AN) is the most severe in terms

of morbidity psychiatric illness with the highest mortality rate

increased by 23 fold. Treatments are limited effectiveness. AN has

a strong genetic component with heritability at 70% but despite

200 studies no major gene was identified. Epigenetics, such

as DNA methylation, is another component of heritability that

could explain the high heritability. Methylation is poorly stud-

ied in AN from small samples, and is focused on few candidate

genes among publications. Under publication, a first genome-wide

methylation study investigated 10 restrictive type AN patients, 19

binging/purging type of AN patients and 15 normal eaters using

DNAs from whole blood (Booij, 2015). Of the 480K CpG sites that

can be methylated of Infinium Human Methylation450 BeadChip

Kit, authors focused on 24,000 sites located close to genes and they

identified candidate genes with a different profile of methylation

between AN and controls.

Objectives

Our work is to replicate the results of Booji and also to

investigate the AN remitters.

Aims

Our goal is to identify epigenetic signatures of the AN dis-

order and the prognostic of remission.

Methods

Twenty-four AN patients, 24 AN remitters will be com-

pared to 48 healthy control women for methylation using the

Infinium Human Methylation450.

Results

As Booji et al., we will compare methylation for 24,000

sites located close to genes for 24 AN, 24 remitters and 48 controls.

Conclusions

We expected to replicate the published results of

Booji and to identify genes with a methylation signature specific

of the AN remission.

Disclosure of interest

The authors have not supplied their decla-

ration of competing interest.

http://dx.doi.org/10.1016/j.eurpsy.2016.01.856

S41

Whole-genome epigenetic changes

genome regarding childhood

maltreatment in patients with

borderline personality disorder or

depression

J. Prados

1

, L. Stenz

2 ,

, P. Courtet

3

, P. Prada

4

, R. Nicastro

5

,

A. Wafae

1

, S. Guillaume

3

, E. Olié

3

, J.M. Aubry

4

, A. Dayer

1

,

N. perroud

4

1

University of Geneva, Department of psychiatry, Geneva,

Switzerland

2

University of Geneva, Department of genetic medicine and

development, Geneva, Switzerland

3

University of Montpellier, Department of mental health and

psychiatry, Montpellier, France

4

University Hospitals of Geneva, Department of mental health and

psychiatry, Geneva, Switzerland

5

University Hospital of Geneva, Department of mental health and

psychiatry, Geneva, Switzerland

Corresponding author.

Early life adversity plays a critical role in the emergence of bor-

derline personality disorder (BPD) and this could occur through

epigenetic programming. In this perspective, we aimed to deter-

mine whether childhood maltreatment could durably modify

epigenetic processes by themeans of a whole-genomemethylation

scanof BPD subjects. Using the Illumina Infinium

®

HumanMethyla-

tion450 BeadChip, global methylation status of DNA extracted from

peripheral blood leucocytes was correlated to the severity of child-

hood maltreatment in 96 BPD subjects suffering from a high level

of child adversity and 93 subjects suffering from major depressive

disorder (MDD) and reporting a low rate of child maltreatment.

Several CpGs within or near the following genes (

IL17RA

,

miR124-

3

,

KCNQ2

,

EFNB1

,

OCA2

,

MFAP2

,

RPH3AL

,

WDR60

,

CST9L

,

EP400

,

A2ML1

,

NT5DC2

,

FAM163A

and

SPSB2

) were found to be differently

methylated, either in BPD compared with MDD or in relation to

the severity of childhood maltreatment. A highly relevant biolog-

ical result was observed for cg04927004 close to miR124-3 that

was significantly associated with BPD and severity of childhood

maltreatment. miR124-3 codes for a microRNA (miRNA) targeting

several genes previously found to be associated with BPD such as

NR3C1. Our results highlight the potentially important role played

bymiRNAs in the etiology of neuropsychiatric disorders such as BPD

and the usefulness of using methylome-wide association studies

to uncover such candidate genes. Moreover, they offer new under-

standing of the impact of maltreatments on biological processes

leading to diseases and may ultimately result in the identification

of relevant biomarkers.

Disclosure of interest

The authors have not supplied their decla-

ration of competing interest.

http://dx.doi.org/10.1016/j.eurpsy.2016.01.857

European alliances against depression: 4-level

interventions targeting depression and suicidal

behaviour

S42

Community-based 4-level approach:

Background, implementation and

evidence for efficacy

U. Hegerl

1 ,

, E. Arensman

2

, C. van Audenhove

3

, T. Baader

4

,

R. Gusmão

5 , A.

Ibelshäuser

6 , Z. M

erali

7 , C. R

ummel-Kluge

8 ,

V. Peréz Sola

9 , R. P

ycha

10 , A.

Värnik

11 , A.

Székely

12

1

University of Leipzig, Department of Psychiatry and Psychotherapy,

Leipzig, Germany

2

NSRF, National Suicide Research Foundation, Cork, Ireland

3

LUCAS, Katholieke Universiteit Leuven, Leuven, Belgium

4

Universidad Austral de Chile Facultad Medicina, Instituto

Neurociencias Clinicas, Valdivia, Chile

5

EUTIMIA, EUTIMIA, Lisbon, Portugal

6

Pro mente tirol, St. Pölten, Austria

7

University of Ottawa, Institute of Mental Health Research, Ottawa,

Canada

8

Stiftung Deutsche Depressionshilfe, University of Leipzig,

Department of Psychiatry and Psychotherapy, Leipzig, Germany

9

Parc de Salut Mar Barcelona, Institut de Neuropsiquiatria i

Addicions, Barcelona, Spain

10

Krankenhaus Bruneck, Abteilung für Psychiatrie, Bruneck, Italy

11

ERSI, Estonian-Swedish Mental Health and Suicidology Institute,

Tallinn, Estonia

12

Semmelweis University, Institute of Behavioural Science, Budapest,

Hungary

Corresponding author.

The community-based 4-level-intervention concept devel-

oped within the “European Alliance against Depression”

( http://www.eaad.net/ )

combines two important aims: to improve

the care and treatment of patients with depression and to prevent

suicidal behavior. It has been shown to be effective concerning the

prevention of suicidal behavior

[1–4] a

nd is worldwide the most

broadly implemented community-based intervention targeting

depression and suicidal behavior. The 4-level intervention concept

comprises training and support of primary care providers (level

1), a professional public relation campaign (level 2), training

of community facilitators (teacher, priests, geriatric caregivers,