European Psychiatry - Volume 33 - March 2016

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24th European Congress of Psychiatry / European Psychiatry 33S (2016) S18–S55 S31
2 Hôpital R.-Debré, Psychiatrie de l’enfant et de l’adolescent, Paris,
France
3 Hôpital Sainte-Anne, CMME, Paris, France
∗ Corresponding author.
Introduction Anorexia nervosa (AN) is the most severe in terms
of morbidity psychiatric illness with the highest mortality rate
increased by 23 fold. Treatments are limited effectiveness. AN has
a strong genetic component with heritability at 70% but despite
∼ 200 studies no major gene was identified. Epigenetics, such
as DNA methylation, is another component of heritability that
could explain the high heritability. Methylation is poorly stud-
ied in AN from small samples, and is focused on few candidate
genes among publications. Under publication, a first genome-wide
methylation study investigated 10 restrictive type AN patients, 19
binging/purging type of AN patients and 15 normal eaters using
DNAs from whole blood (Booij, 2015). Of the 480K CpG sites that
can be methylated of Infinium Human Methylation450 BeadChip
Kit, authors focused on 24,000 sites located close to genes and they
identified candidate genes with a different profile of methylation
between AN and controls.
Objectives Our work is to replicate the results of Booji and also to
investigate the AN remitters.
Aims Our goal is to identify epigenetic signatures of the AN dis-
order and the prognostic of remission.
Methods Twenty-four AN patients, 24 AN remitters will be com-
pared to 48 healthy control women for methylation using the
Infinium Human Methylation450.
Results As Booji et al., we will compare methylation for 24,000
sites located close to genes for 24 AN, 24 remitters and 48 controls.
Conclusions We expected to replicate the published results of
Booji and to identify genes with a methylation signature specific
of the AN remission.
Disclosure of interest The authors have not supplied their decla-
ration of competing interest.
S41
Whole-genome epigenetic changes
genome regarding childhood
maltreatment in patients with
borderline personality disorder or
depression
J. Prados , L. Stenz ∗, P. Courtet , P. Prada , R. Nicastro ,
A. Wafae , S. Guillaume , E. Olié , J.M. Aubry , A. Dayer ,
N. perroud
1 University of Geneva, Department of psychiatry, Geneva,
Switzerland
2 University of Geneva, Department of genetic medicine and
development, Geneva, Switzerland
3 University of Montpellier, Department of mental health and
psychiatry, Montpellier, France
4 University Hospitals of Geneva, Department of mental health and
psychiatry, Geneva, Switzerland
5 University Hospital of Geneva, Department of mental health and
psychiatry, Geneva, Switzerland
∗ Corresponding author.
Early life adversity plays a critical role in the emergence of bor-
derline personality disorder (BPD) and this could occur through
epigenetic programming. In this perspective, we aimed to deter-
mine whether childhood maltreatment could durably modify
epigenetic processes by themeans of a whole-genomemethylation
scanof BPD subjects. Using the Illumina Infinium® HumanMethyla-
tion450 BeadChip, global methylation status of DNA extracted from
peripheral blood leucocytes was correlated to the severity of child-
hood maltreatment in 96 BPD subjects suffering from a high level
of child adversity and 93 subjects suffering from major depressive
disorder (MDD) and reporting a low rate of child maltreatment.
Several CpGs within or near the following genes (IL17RA, miR124-
3, KCNQ2, EFNB1 , OCA2, MFAP2, RPH3AL , WDR60 , CST9L, EP400,
A2ML1, NT5DC2, FAM163A and SPSB2) were found to be differently
methylated, either in BPD compared with MDD or in relation to
the severity of childhood maltreatment. A highly relevant biolog-
ical result was observed for cg04927004 close to miR124-3 that
was significantly associated with BPD and severity of childhood
maltreatment. miR124-3 codes for a microRNA (miRNA) targeting
several genes previously found to be associated with BPD such as
NR3C1. Our results highlight the potentially important role played
bymiRNAs in the etiology of neuropsychiatric disorders such as BPD
and the usefulness of using methylome-wide association studies
to uncover such candidate genes. Moreover, they offer new under-
standing of the impact of maltreatments on biological processes
leading to diseases and may ultimately result in the identification
of relevant biomarkers.
Disclosure of interest The authors have not supplied their decla-
ration of competing interest.
European alliances against depression: 4-level
interventions targeting depression and suicidal
behaviour
S42
Community-based 4-level approach:
Background, implementation and
evidence for efficacy
U. Hegerl ∗, E. Arensman , C. van Audenhove , T. Baader ,
R. Gusmão Ibelshäuser erali ummel-Kluge
V. Peréz Sola ycha Värnik Székely
1 University of Leipzig, Department of Psychiatry and Psychotherapy,
Leipzig, Germany
2 NSRF, National Suicide Research Foundation, Cork, Ireland
3 LUCAS, Katholieke Universiteit Leuven, Leuven, Belgium
4 Universidad Austral de Chile Facultad Medicina, Instituto
Neurociencias Clinicas, Valdivia, Chile
5 EUTIMIA, EUTIMIA, Lisbon, Portugal
6 Pro mente tirol, St. Pölten, Austria
7 University of Ottawa, Institute of Mental Health Research, Ottawa,
Canada
8 Stiftung Deutsche Depressionshilfe, University of Leipzig,
Department of Psychiatry and Psychotherapy, Leipzig, Germany
9 Parc de Salut Mar Barcelona, Institut de Neuropsiquiatria i
Addicions, Barcelona, Spain
10 Krankenhaus Bruneck, Abteilung für Psychiatrie, Bruneck, Italy
11 ERSI, Estonian-Swedish Mental Health and Suicidology Institute,
Tallinn, Estonia
12 Semmelweis University, Institute of Behavioural Science, Budapest,
Hungary
∗ Corresponding author.
The community-based 4-level-intervention concept devel-
oped within the “European Alliance against Depression”
combines two important aims: to improve
the care and treatment of patients with depression and to prevent
suicidal behavior. It has been shown to be effective concerning the
prevention of suicidal behavior nd is worldwide the most
broadly implemented community-based intervention targeting
depression and suicidal behavior. The 4-level intervention concept
comprises training and support of primary care providers (level
1), a professional public relation campaign (level 2), training
of community facilitators (teacher, priests, geriatric caregivers,